ABSTRACT Consumption of ready-to-eat (RTE) vegetables is quick, easy and healthy, especially when eaten without cooking. However, they might be a source of foodborne pathogenic microorganisms. The objective of this study was to evaluate the microbiological and parasitological contamination of fresh RTE vegetables produced in agroindustries in the Federal District of Brazil (FD), and to correlate contamination with good manufacturing practices (GMP). One hundred and three samples of RTE vegetables were collected from six agroindustries for microbiology and parasitology analyses and correlate with GMP; 54 samples were collected from three hypermarkets for parasitological evaluation. None of the samples analyzed were positive for Salmonella sp. and for thermotolerant coliforms, but they were contaminated with total coliforms. All analyzed samples were contaminated with at least one species of enteroparasistes or commensals, which were identified as Ascaris sp., Balantidium coli, Entamoeba histolytica/dispar, Hookworm, Strongyloides sp., Trichuris sp., Entamoeba sp., eggs and larvae of Nematoda, insects and fungi. Agroindustries that adopted GMP showed less contamination with helminths. RTE vegetables sold in hypermarkets of the FD are unfit for human consumption. It is important to guide farmers in the FD on the need to adopt good practices in the production and processing of vegetables to reduce the microbial contamination.

Resumo A couve de folhas é um alimento com alto teor de vitaminas e minerais, que são fundamentais para a dieta humana. Contudo, informações sobre a influência dos fatores extrínsecos e intrínsecos, durante o seu cultivo, em sua composição são escassas. O objetivo deste trabalho foi quantificar os seguintes minerais em folhas de couves produzidas no Distrito Federal (DF): cálcio, magnésio, potássio, fósforo, zinco, manganês, sódio e ferro, correlacionando os teores verificados com os teores presentes no solo. As amostras de folhas e solo foram coletadas em 16 diferentes propriedades rurais no DF, quando também foram aplicados questionários aos produtores sobre as práticas culturais adotadas durante a produção. Os resultados obtidos de teores médios foliares (base em folhas frescas) por 100 g de alimento foram: cálcio, 222,8 mg; ferro, 1,3 mg; magnésio, 37,6 mg, e zinco, 0,6 mg. Esta composição atende a 28% das necessidades diárias de cálcio em crianças de 4 a 8 anos, sendo também supridos 13% de ferro, 29% de magnésio e 13% de zinco, com base no consumo de 100 g de folhas frescas de couve. Foi verificada correlação negativa para teores de magnésio no solo e na folha, com R = -0,69. Para os outros minerais analisados não se verificou correlação significativa. O conteúdo de fósforo e de potássio nos solos foi considerado alto, enquanto que, contrariamente, foram determinadas concentrações baixas de teores de magnésio. O teor de micronutrientes na couve foi considerado baixo e aventa para um manejo inadequado da adubação, fator corroborado pelas respostas dos questionários sobre práticas culturais. Práticas de manejo que levam ao equilíbrio de nutrientes no solo refletem diretamente na interação solo-planta. Recomendações regionais atualizadas de adubação e correção dos solos poderiam auxiliar agricultores e técnicos, refletindo diretamente na obtenção de teores mais equilibrados de micro e macronutrientes em alimentos de origem vegetal.

Abstract Collard is a food with a high content of vitamins and minerals, fundamental for the human diet. However, information on the influence of extrinsic and intrinsic factors in its composition during cultivation is still scarce. The objective of this work was to quantify the minerals in collard leaves produced in the Federal District (DF), Brazil: calcium, magnesium, potassium, phosphorus, zinc, manganese, sodium and iron, correlating with the present soil contents. Leaf and soil samples were collected in 16 different rural properties in the Federal District, in which questionnaires were also applied on the cultural practices adopted during plant growing. The average foliar contents per 100 g of fresh leaves were: calcium, 222.8 mg; iron, 1.3 mg; magnesium, 37.6 mg; and zinc, 0.6 mg. This composition meets 28% of daily calcium requirements in children aged from 4 to 8 years: 13% iron, 29% magnesium and 13% zinc, based on the consumption of 100 g of fresh collard leaves. Negative correlation was verified for magnesium contents in soil and leaf, with R = -0.69. A significant correlation was not verified for the other analyzed minerals. Phosphorus and potassium contents in soils were considered high while, contradictorily, low concentrations of magnesium were found. The foliar micronutrients contents were considered low and suggests for an inadequate management of the fertilization, factor corroborated by the answers of the cultural practices questionnaires. Management practices that lead to nutrient balance in soil reflect directly on soil-plant interaction. Up-to-date regional recommendations on soil fertilization and correction could assist farmers and technicians, directly reflecting the achievement of more balanced levels of micro and macro nutrients in vegetables.

Introduction This study evaluated the presence of pathogenic human parasites on field-grown strawberries in the Federal District of Brazil. Methods A total of 48 samples of strawberries and 48 soil samples from 16 properties were analyzed. Results Contaminated strawberries were detected in 56% of the properties. Schistosoma mansoni, Ascaris lumbricoides or Ascaris suum, Balantidium coli, Endolimax nana, and Entamoeba spp. were detected. Soil was contaminated with Entamoeba spp., Entamoeba coli, Strongyloides spp., Ancylostomatidae, and Hymenolepis nana. Conclusions Producers should be instructed on the safe handling of strawberries in order to reduce the incidence of strawberries that are contaminated with enteroparasites.